On a sunny spring day with a lingering edge of a chill, Thomas Daniels, Ph.D., (above, left) and Richard Falco, Ph.D., (right) step out for a quick jaunt in the tick-rich environs of Fordham’s Louis Calder Center Biological Field Station.

Dragging a 3-foot by 3-foot sheet of white corduroy along the side of the road, they snare a half dozen black legged ticks on the cloth at a rate of more than one per minute.

“They’re resting on top of the blades of grass and waving their front legs, just looking for something to carry them off,” says Daniels, an associate research scientist in the biology department.

Something like a deer, perhaps? The leg of a dog? Or a human ankle? Yes, he says, pretty much anything they can latch onto that will provide a blood meal.

Rich Falco, Ph.D., helps New York state manage insects that are a threat to public health. Photos by Bruce Gilbert

But the ticks that Falco and Daniels catch are headed for an odd form of immortality at Calder’s Routh House, a sprawling, two-story brick structure that hosts Fordham’s Vector Ecology Laboratory (VEL). As co-directors of the VEL, they oversee University-funded research on diseases carried by ticks and mosquitoes.

Some of the ticks will be painted for a mark-release-recapture project. Others will be tested for the presence of Lyme and other diseases. One may end up on a slide as a reference sample. Still others will simply be counted and then stored in ethanol.

Yes, there is state and regional surveillance of New York ticks and mosquitoes, and Falco, Daniels and their staff are the ones who handle it.

Since 2007, the Calder Center has held a contract with the New York State Department of Health to act as its Regional Medical Entomology Laboratory (MEL) for nine counties in the metropolitan region, by far its most populous area.

The contract, which runs through 2011, designates Calder as one of New York’s focal points for vector-borne disease research and related public health issues.

As a state regional medical entomologist, Falco coordinates the sampling of ticks and mosquitoes in the lower Hudson Valley; he is assigned to the counties of Sullivan, Ulster, Duchess, Putnam, Orange, Rockland and Westchester, as well as Nassau and Suffolk in Long Island. He oversees four services:

• conducting routine surveillance on ticks and mosquitoes, as well as the various pathogens that infect them, and reporting that information to the state;

• finding ways to detect and control the risks of diseases carried by ticks and mosquitoes;

• offering instruction to undergraduate and graduate students and physicians; Falco also teaches at New York Medical College;

John Kokas keeps a state insect reference collection.

• providing outreach and assistance to the county health departments on ticks, mosquitoes and other vector-borne diseases, which includes being response-ready in the case of a public outbreak of a disease such as the West Nile virus.

“Perhaps that is our most serious obligation,” Falco said. “If there is a public health emergency of that nature, we would stop whatever we are doing, assist our assigned counties with their trapping efforts and help coordinate the research.”

Such emergencies are rare, he added, but one happened in the summer of 1999, with the sudden outbreak of West Nile virus in all five boroughs and the surrounding region. That year, 62 people became ill and seven people died from a new vector-borne disease spread by mosquitoes that had bitten infected birds. Birds were implicated in the transmission cycle when thousands were found dead in New York. This was the first time West Nile had been reported in North America; today, it is endemic in the United States.

“The area north of New York City is a prime mixing zone between people and vectors,” Falco said. “You’ve got suburban rural habitats where you find ticks and mosquitoes. You’ve got an urban area teeming with people. Everything comes together here.

“The state saw Calder’s location and the VEL’s track record, and realized it was an advantageous spot for a state entomology lab,” he said.

In the Routh House labs, a person can’t wander too far without being confronted by bugs—living, dead, or immortalized in popular culture. The hallway is decorated with movie posters from some of the mutant bug genre classics: Arachnophobia, Tarantula. Sitting atop of two filing cabinets is a “Flea Circus” game, circa 1960.

“Tom picked that up at a flea market,” Falco said jokingly.

Besides employing Falco, the state pays for two research technicians: John Kokas and Vanessa Vinci. Kokas’ area of the lab is inhabited by, among other things, a live tarantula and an aquarium filled with prehistoric-looking Madagascar Hissing Cockroaches.

“I keep them just for fun,” he said.

Tom Daniels, Ph.D.,  studies what makes ticks tick.

Kokas is completely serious, however, when it comes to the business of mosquitoes. In fact, Falco refers to him as “a real bug man.” He is responsible for collecting, identifying and mounting some 40 to 50 different mosquito species found all over the state.

Kokas keeps a New York state insect specimen reference collection in several wooden cabinets housing glass display cases—from the smallest mosquito (Uranotaenia) to the largest (Toxorhynchites) as well as a host of beetles, biting flies, bees, wasps, lice and bed bugs for educational and identification purposes.

When local health departments prepare to do their annual mosquito surveillance each spring, they often send their personnel to the MEL to train with Kokas.

“You need good clean intact specimens in order to train people what to look for,” Kokas said. “So we bring them here to show them the mountings. Once they study them, they can usually go out and I.D. them.”

Through his microscope, Kokas displayed the beautiful side of ugly: up close, an Uranotaenia sapphirina mosquito is ornamented with fine iridescent blue stripes on its body and gossamer wings.

To catch mosquitoes, Kokas uses a wooden box painted black, facing west. When the sun is out, Kokas said, biting mosquitoes go wherever they can to stay in the shade. Trapping for counting purposes is also done with light traps and gravid traps for egg-bearing females.

MEL staffers work closely with the state to monitor medically important insect populations and share that information. Falco reports regularly to Albany on the site totals from his counties. Twice ayear he sends samples of ticks from his counties to be tested for agents of Lyme disease and other tick-borne infections.

Lyme disease is the biggest vector-borne disease in the country, and a quarter of the nation’s cases occur right here in New York state. Approximately 25 percent of the nymphal ticks and 50 percent of the adult ticks that Daniels and Falco encounter are infected with the Lyme disease agent.

“From this surveillance work, we get a handle on what the infection rates are throughout the state and how they are changing over time,” Falco said.

As an additional service to surrounding counties, during the active tick season—May to October—Fordham’s VEL posts a once-weekly Tick Index online. The index, which went active on Memorial Day weekend, estimates the risk of being bitten by a nymphal or adult deer tick based on the density of their population during the week.

Daniels and VEL staffers do research on how disease spreads among ticks, which animals they feed on, and how temperature affects their life cycle; all research conducted by the VEL is also available for use by New York state.

“The beauty of melding the two institutions—Fordham and the regional Medical Entomology Lab—is the way we can join hands,” Falco said. “The state is interested in using our research to reduce risk and maintain public health. While the University, with its academic perspective, is interested in the basic ecology of vector-borne disease systems.”

There is, of course, some slight risk in working with diseased insects, said Falco, who was one of the first scientists to earn a doctorate on the study of the tick known as Ixodes scapularis. Over the years both he and Daniels have contracted Lyme disease, more than just once.

“But that’s the nature of the job,” he said.

The contract, which will run through 2011, designates the Calder Center as one of the state’s focal points for vector-borne disease research and related public health issues. It will cover Sullivan, Ulster, Duchess, Putnam, Orange, Rockland and Westchester counties and parts of Long Island. Previously, the region’s entomology laboratories had been located at Cornell University in Ithaca and Sullivan County Community College, part of the State University of New York system in Loch Sheldrake, N.Y.

Under the contract, the Calder Center will monitor local populations of ticks and mosquitoes; research ways to detect and control the risks of diseases these insects carry; collaborate with local departments of health in all nine counties with regard to such diseases; and be response-ready to public outbreaks of vector-borne diseases such as the West Nile virus. The contract will pay the salaries for researchers and technicians to work side-by-side with staff at the Calder Center’s well-established Vector Ecology Laboratory (VEL).

Richard Falco, Ph.D., associate research scientist and co-director of the VEL, will act as the state’s metropolitan regional medical entomologist. “Given our experienced personnel, the strong commitment by Fordham in supporting this kind of work and our location in a suburban, populated habitat where mosquitoes and ticks proliferate, it was a natural place for the state to choose,” Falco said.

The contract comes on the heels of the Calder Center’s receipt of a $388,000 grant from the National Institutes of Health to study fungi that can kill the black-legged tick, one of the major transmitters of Lyme disease. That grant was awarded to Falco, VEL co-director and associate research scientist Thomas Daniels, Ph.D. and Amy Tuininga, Ph.D., associate professor of biology.

Nancy Busch, dean of the Graduate School of Arts and Sciences (GSAS), said the state has recognized the “quality work done by the staff of the VEL for many years.

“The contract demonstrates the positive benefits of Fordham’s commitment to basic scientific research and applications—both prevention and intervention,” she added.